Human thymidylate synthase
resolution: 3.1 /3 .0Å (low / high salt)
method: molecular replacement
R/Rfree: 0.23/0.28 // 0.22/0.28
space group: C121 / P3121
Analysis of mRNA recognition by human thymidylate synthase (2014) Brunn ND, Dibrov SM, Kao MB, Ghassemian M & Hermann T. Biosci. Rep. 34, 905-913. (PDF)
Bacterial ribosomal RNA helix h44 (aminoglycoside and hygromycin B binding site)
resolution: 2.3Å
method: single-wavelength anomalous diffraction from 5-BrU
R/Rfree: 0.19/0.23
space group: P64
PDB: 3LOA
A model for the study of ligand binding to the ribosomal RNA helix h44 (2010) Dibrov SM, Parsons J & Hermann T. Nucleic Acids Res. 38, 4458-4465. (PDF)
Human thymidylate synthase mRNA regulatory element dimer (non-biological hairpin sequence dimer)
resolution: 1.97Å
method: single-wavelength anomalous diffraction from 5-BrU
R/Rfree: 0.18/0.24
space group: P21
PDB: 3MEI
Structure of an RNA dimer of a regulatory element from human thymidylate synthase mRNA (2011) Dibrov SM, McLean JE & Hermann T. Acta Cryst. D 67, 97-104. (PDF)
Hepatitis C virus internal ribosome entry site subdomain IIa RNA
resolution: 3.0Å
method: fragment molecular replacement
R/Rfree: 0.27/0.30
space group: P212121
PDB: 2NOK
Functional architecture of HCV IRES domain II stabilized by divalent metal ion in the crystal and in solution (2007) Dibrov SM, Johnston-Cox H, Weng YH & Hermann T. Angew. Chem. Int. Ed. 46, 226-229. (PDF)
HCV IRES subdomain IIa RNA complex with benzimidazole viral translation inhibitor
resolution: 2.2Å
method: fragment molecular replacement
R/Rfree: 0.18/0.24
space group: P212121
PDB: 3TZR
Structure of a hepatitis C virus RNA domain in complex with a translation inhibitor reveals a binding mode reminiscent of riboswitches (2012) Dibrov SM, Ding K, Brunn ND, Parker MA, Bergdahl BM, Wyles DL & Hermann T.Proc. Natl. Acad. Sci. USA 109, 5223-5228. (PDF)
Seneca Valley Virus internal ribosome entry site subdomain IIa RNA
resolution: 1.86 /3.10Å (2 constructs)
method: fragment molecular replacement
R/Rfree: 0.19/0.23 // 0.21/0.27
space group: H3 / P6322
Functional conservation despite structural divergence in ligand responsive RNA switches (2014) Boerneke MA, Dibrov SM, Gu J, Wyles DL & Hermann T. Proc. Natl. Acad. Sci. USA 111,15952-15957. (PDF)
Crystal structure of an RNA nano triangle
resolution: 2.60Å
method: molecular fragment replacement
R/Rfree: 0.19/0.24
space group: R32
PDB: 5CNR
Crystal structure-guided design of self-assembling RNA nanotriangles (2016) Boerneke MA, Dibrov SM & Hermann T. Angew. Chem. Int. Ed. 55, 4097-4100.
